Officials say Officer Molly Perry, a three-year veteran of the force, and Anne Malone of Branford collided vehicles on the off-ramp of exit 52 around 7 p.m.
Police say Officer Perry was responding to an emergency call when the crash took place.
The officer and the driver of the other vehicle were transported to Yale-New Haven Hospital with non-life threatening injuries.
It is not yet known which vehicle caused the crash.
The incident remains under investigation.